DNA Extraction and Purification
Research Products International Corp DNA Clean & Concentrator-5 w/Zymo-Spin I Columns, Uncapped, 200 preps
The DNA Clean & Concentrator-5 is a PCR purification kit that provides purification of up to 5 µg DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc. This product facilitate the removal of DNA polymerases, modifying enzymes, RNA polymerases, ligases, kinases, nucleases, phosphatases, and restriction endonucleases, as well as free dNTPs and their analogs including radiolabeled and fluorescent derivatives. Eluted DNA is suitable for PCR, arrays, ligation, sequencing, etc.
Clean and concentrate up to 5 µg DNA with ≥ 6 µl elution in as little as 2 minutes with 0 µl wash residue carryover.
Column design allows DNA to be eluted at high concentrations into minimal volumes of water or TE buffer.
Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc.
Research Products International Corp EZ DNA Methlyation-Lightning Kit, 200 Reactions
The EZ DNA Methylation-Lightning Kit can rapidly bisulfite convert and purify DNA in less than 1.5 hours. A ready-to-use conversion reagent streamlines this process – simply add the reagent directly to the sample and incubate. Desulphonation and clean-up of the converted DNA is performed on a spin-column, allowing an elution volume as low as 10 µl. The kit achieves high conversion efficiency of unmodified cytosines into uracil, ensuring accurate downstream methylation analysis.
Fastest bisulfite conversion kit for complete bisulfite conversion of DNA for methylation analysis.
Ready-to-use conversion reagent is added directly to DNA.
High-yield, converted DNA is ideal for PCR, Methylation Specific PCR (MSP), arrays, library preps, Next-Generation sequencing, etc.
Sigma Aldrich Fine Chemicals Biosciences DNase I Amplification Grad
Deoxyribonuclease I (DNase I) is an endonucleaseisolatedfrom bovine pancreas that digests double and single stranded DNA into oligo and mononucleotides. Amplification Grade DNase I has been purified to remove RNase activity and is suitable for eliminating DNA from RNA preparations prior to sensitive applications such as RT-PCR (Reverse Transcriptase - Polymerase Chain Reaction). DNase I digests double- and single-stranded DNA into oligo- and mononucleotides. Using the Reaction Buffer provided DNA is removed from RNA preparations in a 15 minute digestion at room temperature. The DNase I is then inactivated by heating with the Stop SOLUTIONon. Heating also denatures hairpins in the RNA so the RNA can be used directly in reverse transcription.No current RNA isolation procedure removes 100% of the DNA. Many commercial DNase I formulations are contaminated with residual RNases. This RNase contamination can destroy or degrade valuable RNA samples prior to reverse transcription. Laborator
Epoch Life Science Inc GenCatch™ Plant Genomic DNA Purification System (250 preps) Uniquely formulated buffers, shearing tubes, reinforced columns with collection tubes, along with protocols.
Provides a simple, fast and cost-effective method to extract gDNA ready for downstream use from various plant species. Comes with shearing tubes for simple and fast homogenization and filtration of plant tissues. 40 ug yield. 100 mg plant tissue. 200 ul elution volume. Designed predominantly for gDNA of 20-30 kb. No phenol/chloroform extraction nor ethanol precipitation required. Used by labs around the world. 18 month shelf life.
Epoch Life Science Inc GenCatch™ Anion Exchange DNA Maxi Columns (50/PK)
Ion exchange columns for transfection grade plasmid DNA purification. Anion-exchange technology. 1000 ug (1 mg) plasmid DNA binding capacity. Gravity flow. Compatible with third party buffer sets.
Epoch Life Science Inc GenCatch™ Plasmid DNA Midiprep Kit (50 preps) Uniquely formulated buffers, reinforced anion exchange columns with collection tubes, along with protocols.
Provides a simple, fast and cost-effective method to purify transfection grade plasmid DNA using ion (anion) exchange technology without phenol extraction or ethanol precipitation. 100 ug plasmid DNA binding capacity. Gravity flow. DNA prepared by this kit is suitable for many downstream molecular manipulations including PCR, ligation, restriction enzyme digestion, transformation and transfection, radioactive and fluorescent sequencingg, etc.
Research Products International Corp Quick-DNA Fungal/Bacterial DNA MiniPrep Kit, 50 Preps
The Quick-DNA Fungal/Bacterial Miniprep Kit is designed for the simple and rapid isolation of DNA from tough-to-lyse fungi, including A. fumigatus, C. albicans, N. crassa, S. cerevisiae, S. pombe, as well as Gram (+/-) bacteria, algae, and protozoa. The procedure is easy and can be completed in minutes: fungal and/or bacterial samples are rapidly and efficiently lysed with our state of the art, ultra-high density BashingBeads. Zymo-Spin column technology is then used to isolate the DNA that is ideal for downstream molecular-based applications including PCR, array, etc.
Boost Detection: Included BashingBeads ensure complete lysis of tough-to-lyse samples.
Ultra-Pure: Ready for qPCR, Next-Gen Sequencing, arrays, etc.
Simple: Fastest workflow (≤ 20 minutes).
Research Products International Corp ZR Plasmid Miniprep-Classic Kit, 100 Preps
The ZR Plasmid Miniprep-Classic is designed for efficient isolation of plasmid DNA from E. coli using a traditional 3-buffer (P1, P2, P3) procedure that is simple, rapid, and reliable. It features a modified alkaline lysis protocol together with Zymo-Spin technology to yield high quality plasmid DNA in minutes. The buffers are color-coded (red, green, yellow) for easy visualization of complete cell lysis and neutralization. The innovative Zymo-Spin llN columns yield transfection-grade plasmid DNA. Plasmid DNA purified using the ZR Plasmid Miniprep-Classic is well suited for use in restriction endonuclease digestion, sequencing, DNA ligation, cloning, PCR, bacterial transformation, transfection, etc.
High Purity: Eluted plasmid DNA is high quality, endotoxin-free and ready for all sensitive downstream applications including transfections and sequencing.
Colored Buffers: P1, P2, and P3 buffers are colored for easy visualization of complete lysis and neutralization.
Norgen Biotek Corp Urine DNA Isolation Micro Kit
This kit provides a fast, reliable and simple procedure for isolating DNA from up to 1.75 mL of urine. Both high molecular weight DNA (greater than 1 kb in size; mostly cell associated) and the smaller DNA (150 - 250 bp; derived from the circulation) is effectively isolated and purified using a rapid and convenient spin column protocol. Multiple samples can be processed in 30 minutes. This kit can be used to isolate DNA from a broad range of viruses in urine as well. Salts, metabolic wastes, proteins and other contaminants are removed to yield inhibitor-free DNA for use in sensitive applications. The DNA is of excellent quality for various downstream applications such as PCR, qPCR and DNA fingerprinting, methylation studies and more. This kit is fully compatible with Norgen's Urine Collection and Preservation Tubes.
LGC GENOMICS LLC (Lucigen) MasterPure Complete DNA & RNA Purification Kit, 10 Purif
The MasterPure™ Complete DNA and RNA Purification Kit enables rapid, high yield purification of high-molecular-weight genomic DNA, total cellular RNA and Total Nucleic Acid (TNA) from many different sample types.
Research Products International Corp Oligo Clean & Concentrator, 200 Preps
The Oligo Clean & Concentrator provides a streamlined method for efficient recovery and clean-up of DNA/RNA fragments and oligonucletides ≥ 16 nt from labeling (radioactive, biotin, DIG, etc.) and other enzymatic reactions. Unincorporated nucleotides, short oligos, dyes, enzymes, and salts are effectively removed by the clean-up procedure. DNA/RNA is washed and concentrated into a small volume of water. Purified DNA/RNA, available in just 2 minutes, is suitable for hybridization, gel shift assays, enzymatic reactions, ligation, sequencing, microarray analysis, etc.
Fast & Simple: Clean and concentrate DNA and RNA oligonucleotides in 2 minutes.
Ultra-Pure: Ready for hybridization, Next-Gen Sequencing, PCR, ligations, etc.
Complete removal: Eliminate dyes, salts, enzymes, nucleotides, and short oligos.
Research Products International Corp Zyppy Plasmid Miniprep Kit, 400 Preps
The Zyppy Plasmid Miniprep Kit is a plasmid DNA kit that features a pellet-free modified alkaline lysis system that bypasses the normal cell-pelleting and resuspension steps common to classical plasmid preparation procedures. Simply add the uniquely formulated 7X Lysis Buffer directly to your bacterial culture, neutralize, and then purify with Zymo-Spin column technology. Additionally, the patented colored buffers included in the kit allow for easy visualization of complete cell lysis and neutralization. The Zyppy Plasmid Miniprep Kit is the fastest and easiest method available to separate plasmid DNA from E. Coli efficiently. The plasmid DNA is high quality, endotoxin-free, and well suited for use in transfection, bacterial transformation, restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications.
Fastest: 8 minutes from culture flask to high-quality plasmid DNA.